Genome sequencing of the neotype strain CBS 554.65 reveals the MAT1–2 locus of Aspergillus niger

Abstract Background Aspergillus niger is a ubiquitous filamentous fungus widely employed as cell factory thanks to its abilities produce wide range of organic acids and enzymes. Its genome was one the first genomes be sequenced in 2007, due economic importance role model organism study fungal fermentation. Nowadays, sequences more than 20 A. strains are available. These, however, do not include neotype strain CBS 554.65. Results The 554.65 with PacBio. A high-quality nuclear sequence consisting 17 contigs N50 value 4.07 Mbp obtained. assembly covered all 8 centromeric regions chromosomes. In addition, complete circular mitochondrial DNA Bioinformatic analyses revealed presence MAT1-2-1 gene this genome, contrary most commonly used strains, such ATCC 1015 513.88, which contain MAT1-1-1 gene. nucleotide alignment showed different orientation MAT1–1 locus compared MAT1–2 554.65, relative conserved genes flanking MAT locus. Within 24 newly isolates half them had other genomic organization similar species. contrast, region comprising flipped . Conclusions This study, besides providing an important strain, suggests occurrence genetic flipping or switching events at niger. These results provide new insights mating system could contribute investigation potential discovery sexuality species long thought asexual.